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By the time Sanger sequencing was commercialized, the groundwork for an entirely new sequencing chemistry was already well underway. In 1985, Swedish biochemists Pål Nyren and Arne Lundin published a paper illustrating a procedure that measured the concentration of a molecule, called pyrophosphate (PPi), using an enzymatic cascade that emits light. In early 1986, Nyren realized that the method he’d helped develop could be applied to DNA sequencing, because PPi is naturally produced as a byproduct of DNA synthesis.5

红牛在过去的 5 年里,获得了 2 个制造商冠军和 4 个车手冠军,从两种冠军的数量差异你应该可以猜到,这支车队似乎有一位车手存在感比较弱,没拿到太多积分。

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