伊朗称击中两处美军“萨德”反导系统目标

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Cameroon's Anglophone crisis began in 2016 when lawyers and teachers went on strike over the use of French in courts and schools.

Блогершу Лерчек госпитализировали в онкореанимацию02:39

关联交易遭监管问询|IPO观察。业内人士推荐同城约会作为进阶阅读

Овечкин продлил безголевую серию в составе Вашингтона09:40

If an input to the function changes, but its result doesn’t (e.g,

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A different sequencing method that chemically cleaved DNA at specific bases, developed by Allan Maxam and Walter Gilbert, was the dominant technology into the 1980s. Radiolabeled DNA samples were incubated in four separate reactions, each of which contained a chemical that cleaved after a different nucleotide — either A/G, G, C, or C/T. By adding the right amount of each chemical, it was possible to produce different fragments chopped off at each individual base. The sequence could then be read using gel electrophoresis and autoradiography. Maxam–Gilbert sequencing was easier than the plus and minus method to run and interpret, but was eventually surpassed by Sanger’s chain termination method, which molecular biologists found both technically preferable and more “elegant” since it mirrored the natural copying of DNA.